| Product Name | ADAM12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADAM12ADAM12 is a member of the ADAM (a disintegrin and metalloprotease-like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine-rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine-rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin-processed form. |
| Immunogen | A synthetic peptide corresponding to the cytoplasmic domain of human ADAM12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000 - 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 99.5 kDa). Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADAM12 8038 MLTN MCMP MLTNA O43184 MCMPMltna 602714 |
|---|---|
| Target | ADAM12 |
| Tissue Specificity | This antibody may be used for the detection and localization of human ADAM12 and does not react with other ADAMs. Recognizes the full length, long form of ADAM12 but not the soluble form of ADAM12. By immunoblotting against the reduced protein, the antibody recognizes bands of 116 kDa (minor band), 92 kDa (major band), and cleaved products at 68 kDa and 40 kDa in cell lysates. |
| Cellular Localization | Type I membrane protein (isoform 12L). A secreted form (isoform 12S) is produced by alternative splicing. |
| Application Notes | WB: Use at a dilution of 1/1000 - 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 99.5 kDa). Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS |
