WB
WB analysis using ADAM12 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with ADAM12 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human breast cancer using ADAM12 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer using ADAM12 antibody at dilution of 1:50 (under 10x lens).IP
IP experiment of HeLa using ADAM12 Polyclonal antibody. IP result of anti-ADAM12 (4ug; 1:300) with HeLa cells lysate 4650ug.ICC/IF
IF Staining of HepG2 using ADAM12 Polyclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ADAM12 antibody at dilution of 1:100 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).FC
FC experiment of HeLa using ADAM12 Polyclonal antibody. 1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADAM12 Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | ADAM12 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ADAM12 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAM12 |
|---|---|
| Gene Synonyms | MCMP MLTN CAR10 MLTNA MCMPMltna ADAM12-OT1 |
| Gene Full Name | ADAM metallopeptidase domain 12 |
| Gene Summary | This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014] |
| Alternative Names | ADAM 12 Disintegrin and metalloproteinase domain-containing protein 12 EC:3.4.24.- MCMP Meltrin-alpha |
| Molecular Weight(MW) | 100 kDa(Observed band size:90-100 kDa, 70-80 kDa) |
| Cellular Localization | Cell membrane,Membrane,Secreted. |
WB
WB analysis using ADAM12 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with ADAM12 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human breast cancer using ADAM12 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer using ADAM12 antibody at dilution of 1:50 (under 10x lens).
IP
IP experiment of HeLa using ADAM12 Polyclonal antibody. IP result of anti-ADAM12 (4ug; 1:300) with HeLa cells lysate 4650ug.
ICC/IF
IF Staining of HepG2 using ADAM12 Polyclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ADAM12 antibody at dilution of 1:100 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC
FC experiment of HeLa using ADAM12 Polyclonal antibody. 1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADAM12 Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
