Application
Immunocytochemical staining of TACE in HeLa cells with TACE antibody at 10μg/ml.Application
Western blot analysis of ADAM-17 in HeLa (1,3) and Jurkat (2,4) whole cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with Anti-ADAM-17 (TACE) at 1:500 dilution.| Product Name | ADAM17 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ADAM17Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association (6). |
| Immunogen | Peptide sequence - amino acids 807 - 823 of human TACE. (This sequence differs from mouse and rat TACE by one amino acid) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC71942 6868 cSVP P78536 CD156b TACE ADAM17 603639 |
|---|---|
| Target | ADAM17 |
| Tissue Specificity | Recognizes human, mouse, and rat TACE. 80-130 kD bands are detected in immunoblots. These bands may represent mature protein, precursor, and glycosylated TACE. |
| Cellular Localization | Type I membrane protein. |
Application
Immunocytochemical staining of TACE in HeLa cells with TACE antibody at 10μg/ml.
Application
Western blot analysis of ADAM-17 in HeLa (1,3) and Jurkat (2,4) whole cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with Anti-ADAM-17 (TACE) at 1:500 dilution.| Positive Control | HeLa whole cell lysate, Jurkat whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 contianing 0.02% sodium azide |
