Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
ADAM17 antibody [C2C3], C-term detects ADAM17 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract | 7.5% SDS-PAGE ADAM17 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ADAM17 antibody [C2C3], C-term detects ADAM17 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: ADAM17 antibody [C2C3], C-term dilution: 1:250.| Product Name | ADAM17 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ADAM17 (ADAM metallopeptidase domain 17)This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ADAM17. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, ChickenDog (92%), Pig (93%), Chicken (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADAM18 antibody CD156B antibody CSVP antibody MGC71942 antibody TACE antibody ADAM17 antibody TNF-alpha converting enzyme antibody snake venom-like protease antibody tumor necrosis factor alpha converting enzyme antibody TNF-alpha convertase ant |
|---|---|
| Molecular Weight(MW) | 93 kDa (note) |
| Cellular Localization | Membrane |
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
ADAM17 antibody [C2C3], C-term detects ADAM17 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract | 7.5% SDS-PAGE ADAM17 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ADAM17 antibody [C2C3], C-term detects ADAM17 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: ADAM17 antibody [C2C3], C-term dilution: 1:250.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
