| Product Name | ADAM19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADAM19ADAM19, is a member of the ADAM (a disintegrin and metalloprotease-like domain) family. It has been cloned from mouse and human. ADAM19 was first described in muscle cells as a protein with homology to the fertilins (ADAMs 1 and 2). Initial observations indicated a role for ADAM19 in myoblast fusion, similar to sperm-egg fusion aided by ADAMs 1 and 2. ADAM19 may be important in osteoblast differentiation, as a marker for dendritic cell differentiation, and in the intracellular processing of neuregulin. The ADAMs proteins are structurally similar, possessing a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cysteinrich domain, EGF-like repeat, type-I transmembrane domain, and cytoplasmic domain. |
| Immunogen | Synthetic peptide corresponding to the amino end of the propeptide domain (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. This concentration is determined for tissue or cell lysates with an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting dilution of 1/5000 of the antibody is recommended for chemiluminescent substrates. Under reducing conditions, detects bands at 95 kDa, 84 kDa (major band), and breakdown products at 50 kDa and 34 kDa from cell lysates. A strong band at 30 kDa is often seen, which is probably the propeptide domain cleaved after furin activation of ADAM19. Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is necessary to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 8728 ADAM19 MADDAM Q9H013 FKSG34 MLTNB 603640 |
|---|---|
| Target | ADAM19 |
| Cellular Localization | Type I membrane protein. |
| Application Notes | WB: Use at a dilution of 1/1000. This concentration is determined for tissue or cell lysates with an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting dilution of 1/5000 of the antibody is recommended for chemiluminescent substrates. Under reducing conditions, detects bands at 95 kDa, 84 kDa (major band), and breakdown products at 50 kDa and 34 kDa from cell lysates. A strong band at 30 kDa is often seen, which is probably the propeptide domain cleaved after furin activation of ADAM19. Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is necessary to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS with 50% Glycerol |
