| Product Name | ADAMTS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADAMTS2ADAMTS2 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS2 (A Disintegrin And Metalloproteinase with ThromboSpondin-2 motif), also known as Procollagen I N-Proteinase (PNP), was first described in calf skin as a proteinase that processes the amino end of Type-I collagen. PNP expression was found in skin, aorta, liver, tendon, bladder, retina, and skeletal muscle. Later, PNP was found to be a member of a larger family of ADAMs metalloproteinases containing thrombospondin (TS) repeats. Full length human ADAMTS2 contains 1211 amino acids (bovine, 1205 amino acids) and has a predicted mass of 134.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS2 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS2 resolves at a lower molecular weight of 107 kDa, due to cleavage at the furin site. ADAMTS2 contains the canonical HexxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving procollagen. In addition to the metalloprotease domain, ADAMTS2 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, and three thrombospondin 1 like domains, followed by a unique C-terminal domain. ADAMTS2 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein, much of which binds to the ECM (extracellular matrix). ADAMTS2 knockout mice develop fragile skin (similar to dermatospaxis), and male infertility. Mutations of the ADAMTS2 gene are responsible for human Ehlers-Danlos syndrome type VII C and bovine dermatosparaxis. ADAMTS2 is involved in collagen biosynthesis and may also play role in development and angiogenesis. |
| Immunogen | Synthetic peptide corresponding to the C-terminal (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. This dilution is determined for a tissue or cell lysate using an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting antibody dilution of 1/5000 is recommended for chemiluminescent substrates. This antibody, under reducing conditions, identifies the zymogen form at 130-140 kDa, activated forms at 94-105 kDa (major bands), and breakdown products at 50 kDa, 34 kDa in cell culture media and lysates. Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 225410 9509 PCINP ADAMTS-3 O95450 ADAMTS2 hPCPNI NPI ADAM-TS2 PCPNI ADAMTS3 ADAMTS 3 ADAM TS2 |
|---|---|
| Target | ADAMTS2 |
| Cellular Localization | Secreted. Associated with the extracellular matrix (by similarity). |
| Application Notes | WB: Use at a dilution of 1/1000. This dilution is determined for a tissue or cell lysate using an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting antibody dilution of 1/5000 is recommended for chemiluminescent substrates. This antibody, under reducing conditions, identifies the zymogen form at 130-140 kDa, activated forms at 94-105 kDa (major bands), and breakdown products at 50 kDa, 34 kDa in cell culture media and lysates. Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS with 50% Glycerol |
