| Product Name | ADAMTS4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADAMTS4A disintegrin like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS4 or aggrecanase 1) is a member of the ADAMTS family of multidomain proteins that has demonstrated metalloproteinase functions. The ADAMTS4 protein contains a prohormone convertase cleavage site, thrombospondin 1 like domain, cysteine rich domain, and a propeptide domain. ADAMTS4 activity had been shown to be blocked by TIMP1, 2, 3, and 4. |
| Immunogen | Synthetic peptide corresponding to the propeptide domain (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. A starting antibody dilution of 1/5000 is recommended for chemiluminescent substrates. Higher antibody dilutions may be necessary for non-human samples. Full length ADAMTS4 (837 amino acids) has a predicted mass of 90kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS4 a greater apparent molecular weight under reducing conditions. The antibody detects the zymogen form at 98 kDa, activated forms at 64 kDa (major bands), and breakdown products at 30 kDa. A band at approximately 29 kDa is detected in cell culture media, which may be the propeptide domain shed after furin cleavage. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 9507 ADAMTS-2 ADAMTS4 ADMP1 ADMP-1 KIAA0688 ADAMTS2 O75173 ADAMTS-4 603876 ADMP 1 ADAMTS 2 ADAMTS 4 |
|---|---|
| Target | ADAMTS4 |
| Cellular Localization | Secreted. Associated with the extracellular matrix. |
| Application Notes | WB: Use at a dilution of 1/1000. A starting antibody dilution of 1/5000 is recommended for chemiluminescent substrates. Higher antibody dilutions may be necessary for non-human samples. Full length ADAMTS4 (837 amino acids) has a predicted mass of 90kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS4 a greater apparent molecular weight under reducing conditions. The antibody detects the zymogen form at 98 kDa, activated forms at 64 kDa (major bands), and breakdown products at 30 kDa. A band at approximately 29 kDa is detected in cell culture media, which may be the propeptide domain shed after furin cleavage. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS with 50% Glycerol |
