Application
Western blot of ADCY4 using unpurified ADCY4 antibody 1:500 (Lane 1) and with 1:500 dilution (Lane 2)| Product Name | ADCY4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADCY4The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier. Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCrs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that in turn can regulate various metabolic pathways, gene expression, and affect memory functions etc. The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi hetero-trimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of of GDP to GTP that is bound to G protein. The GTP binding reduces the affinity of Gs to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase. The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes. Adenylate cyclase Type 4 is located on chromosome type 11. |
| Immunogen | Synthetic peptide unique to PAC4 protein was used to generate antibodies in rabbit. All peptides were amidated before conjugation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500. Predicted molecular weight: 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Adenylate cyclase 4 Adenylate cyclase type IV Adenylyl cyclase ATP pyrophosphate lyase |
|---|---|
| Target | ADCY4 |
| Cellular Localization | Integral membrane protein |
Application
Western blot of ADCY4 using unpurified ADCY4 antibody 1:500 (Lane 1) and with 1:500 dilution (Lane 2)| Application Notes | WB: Use at a dilution of 1/500. Predicted molecular weight: 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide |
