Application
Sample (50 ug of whole cell lysate) A: Mouse brown adipose tissue 12% SDS PAGE diluted at 1:1000Application
Adenylate kinase 1 antibody [N1C3] detects Adenylate kinase 1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. Adenylate kinase 1 antibody [N1C3] dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:500| Product Name | Adenylate kinase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Adenylate kinase 1 (adenylate kinase 1)Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Chicken, Sheep, BovineRat (90%), Xenopus laevis (80%), Dog (97%), Chicken (86%), Sheep (96%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AK1 antibody ATP-AMP transphosphorylase 1 antibody adenylate kinase isoenzyme 1 antibody AK 1 antibody myokinase antibody adenylate kinase 1 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: Mouse brown adipose tissue 12% SDS PAGE diluted at 1:1000
Application
Adenylate kinase 1 antibody [N1C3] detects Adenylate kinase 1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. Adenylate kinase 1 antibody [N1C3] dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:500| Positive Control | A549 , HCT116 , mouse brown adipose tissue |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
