Application
Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: Raji 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using AK3L1 antibody at 1:100 dilution.Application
Adenylate kinase 3 antibody [N1C3] detects Adenylate kinase 3 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Adenylate kinase 3 protein stained by Adenylate kinase 3 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Adenylate kinase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Adenylate kinase 3 (adenylate kinase 3-like 1)This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (89%), Rat (88%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AK3 antibody AK3L1 antibody AK3L2 antibody MGC166959 antibody AK4 antibody adenylate kinase 3-like 1 antibody ATP-AMP transphosphorylase antibody mitochondrial adenylate kinase-3 antibody GTP:AMP phosphotransferase antibody nucleoside-triphosphate |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: Raji 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using AK3L1 antibody at 1:100 dilution.
Application
Adenylate kinase 3 antibody [N1C3] detects Adenylate kinase 3 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Adenylate kinase 3 protein stained by Adenylate kinase 3 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
