Application
Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ADH5 Antibody (Center) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.Application
Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ADH5 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADH5 (Internal)This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human ADH5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Alcohol dehydrogenase 5 GSH-FDH ADH5 S(hydroxymethyl)glutathione dehydrogenase Alcohol dehydrogenase5 GSHFDH S-(hydroxymethyl)glutathione dehydrogenase Alcohol dehydrogenase classIII FALDH Alcohol dehydrogenase class chi chai Alcohol dehydrogenas |
|---|---|
| Target | ADH5 |
| Molecular Weight(MW) | 39.724 kDa (note) |
Application
Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ADH5 Antibody (Center) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.
Application
Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
