Application
Flow cytometric analysis of K562 cells using ADH5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ADH5 using rabbit polyclonal ADH5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ADH5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, FALDH, FDH, GSH-FDH, 111-, S-(hydroxymethyl)glutathione dehydrogenase, ADH5 ( |
| Product description | ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.1) Martins-de-Souza,D., et.al., Eur Arch Psychiatry Clin Neurosci 259 (3), 151-163 (2009) |
| Immunogen | This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the Central region of human ADH5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADH5 |
|---|---|
| Alternative Names | Alcohol dehydrogenase class-3 Alcohol dehydrogenase 5 Alcohol dehydrogenase class chi chain Alcohol dehydrogenase class-III Glutathione-dependent formaldehyde dehydrogenase FALDH FDH GSH-FDH 111- S-(hydroxymethyl)glutathione dehydrogenase ADH5 ( |
| Molecular Weight(MW) | 40 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rb |
Application
Flow cytometric analysis of K562 cells using ADH5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ADH5 using rabbit polyclonal ADH5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
