Application
Western blot analysis of AFMID Antibody (N-term) in mouse liver tissue lysates (35ug/lane). AFMID (arrow) was detected using the purified Pab.Application
AFMID Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AFMID antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AFMID (N-term)Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites. |
| Immunogen | KLH conjugated synthetic peptide between 63~92 amino acids from the N-terminal region of human AFMID. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AFMID, Probable arylformamidase, Kynurenine formamidase, KF |
|---|---|
| Target | AFMID |
| Molecular Weight(MW) | 33.992 kDa (note) |
| Cellular Localization | Predominantly cytosolic. Some fraction is nuclear. |
Application
Western blot analysis of AFMID Antibody (N-term) in mouse liver tissue lysates (35ug/lane). AFMID (arrow) was detected using the purified Pab.
Application
AFMID Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
