Rabbit polyclonal antibody to Aggrecan (aggrecan)This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Aggrecan. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Dog, Pig, BovineMouse (96%), Rat (96%), Dog (97%), Pig (96%), Bovine (94%)
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
AGC1 antibody, AGCAN antibody, CSPG1 antibody, CSPGCP antibody, MSK16 antibody, SEDK antibody, ACAN antibody, cartilage-specific proteoglycan core protein antibody, large aggregating proteoglycan antibody, chondroitin sulfate proteoglycan core protein 1 an
Molecular Weight(MW)
250 kDa (note) (3)
Application
Application
IHC-P ImageImmunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Aggrecan antibody at 1:100 dilution.
Application
WB ImageSample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:500
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.