Application
AGR2 antibody detects AGR2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with AGR2 antibody at a dilution of 1:1000.Application
AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGR2 protein stained by AGR2 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of AGR2 protein from MCF-7 whole cell extracts using 5 μg of AGR2 antibody . Western blot analysis was performed using AGR2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
AGR2 antibody detects AGR2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. AGR2 antibody diluted at 1:500.| Product Name | AGR2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AGR2 (anterior gradient 2 homolog (Xenopus laevis)) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AGR2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (93%), Pig (96%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GOB-4 , AG2 , anterior gradient 2 homolog (Xenopus laevis) , PDIA17 , HAG-2 , XAG-2 |
|---|---|
| Target | AGR2 |
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Secreted |
Application
AGR2 antibody detects AGR2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with AGR2 antibody at a dilution of 1:1000.
Application
AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGR2 protein stained by AGR2 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of AGR2 protein from MCF-7 whole cell extracts using 5 μg of AGR2 antibody . Western blot analysis was performed using AGR2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
AGR2 antibody detects AGR2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. AGR2 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
