Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AHR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
AHR antibody detects AHR protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AHR protein stained by AHR antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AHR antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
AHR antibody detects AHR protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. AHR antibody diluted at 1:500.Application
Immunoprecipitation of AHR protein from 293T whole cell extracts using 5 μg of AHR antibody . Western blot analysis was performed using AHR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
AHR antibody detects AHR protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AHR antibody diluted at 1:500.| Product Name | AHR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AHR (aryl hydrocarbon receptor)This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AHR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, Bovine, Guinea pigPig (85%), Rabbit (87%), Bovine (86%), Guinea pig (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | bHLHe76 antibody, AHR antibody, ah receptor antibody, class E basic helix-loop-helix protein 76 antibody, aryl hydrocarbon receptor antibody, AH-receptor antibody, aromatic hydrocarbon receptor antibody |
|---|---|
| Molecular Weight(MW) | 96 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AHR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
AHR antibody detects AHR protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AHR protein stained by AHR antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AHR antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
AHR antibody detects AHR protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. AHR antibody diluted at 1:500.
Application
Immunoprecipitation of AHR protein from 293T whole cell extracts using 5 μg of AHR antibody . Western blot analysis was performed using AHR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
AHR antibody detects AHR protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AHR antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
