Application
Immunocytochemical staining of K562 cells with Rabbit anti AIFApplication
Western blot analysis of AIF in K562 cell lysate with AIF antibody at (A) 0.5, (B)1, and (C)2 μg/ml.| Product Name | AIF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AIFApoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and widely expressed (2). |
| Immunogen | Synthetic peptide: amino acids 593-606 of Human AIF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500-1:2,000. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 300169, MGC111425, PDCD8, 9131, O95831, AIF |
|---|---|
| Target | AIF |
Application
Immunocytochemical staining of K562 cells with Rabbit anti AIF
Application
Western blot analysis of AIF in K562 cell lysate with AIF antibody at (A) 0.5, (B)1, and (C)2 μg/ml.| Application Notes | For WB: Use at a dilution of 1:500-1:2,000. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
