Application
Western blot analysis of extracts of various cell lines, using AIFM1 antibody.Application
Immunofluorescence analysis of HeLa cell using AIFM1 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of U2OS cell using AIFM1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human esophageal using AIFM1 antibody at dilution of 1:100 (x400 lens).| Product Name | AIF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AIFThis gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010] |
| Immunogen | Recombinant protein of human AIFM1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | apoptosis-inducing factor, mitochondrion-associated, 1 , COWCK , CMTX4 , PDCD8 , COXPD6 , AIFM1 |
|---|---|
| Target | AIF |
| Molecular Weight(MW) | 67 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using AIFM1 antibody.
Application
Immunofluorescence analysis of HeLa cell using AIFM1 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of U2OS cell using AIFM1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human esophageal using AIFM1 antibody at dilution of 1:100 (x400 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
