Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term diluted at 1:4000.Application
AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: AIFM1 protein stained by AIF antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using AIF antibody at 1:100 dilution.Application
AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE AIF antibody [N1N2], N-term dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AIF antibody at 1:100 dilution.| Product Name | AIF antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AIF (apoptosis-inducing factor, mitochondrion-associated, 1)This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human AIF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIF antibody, COXPD6 antibody, MGC111425 antibody, PDCD8 antibody, AIFM1 antibody, striatal apoptosis-inducing factor antibody, apoptosis-inducing factor 1, mitochondrial antibody, programmed cell death 8 (apoptosis-inducing factor) antibody, apoptosis-ind |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (2) |
| Cellular Localization | Mitochondrion intermembrane space , Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term diluted at 1:4000.
Application
AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: AIFM1 protein stained by AIF antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using AIF antibody at 1:100 dilution.
Application
AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE AIF antibody [N1N2], N-term dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AIF antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
