Application
Sample(30 μg of whole cell lysate) A:H1299 B:Hep G2 12% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
AIMP1 antibody detects SCYE1 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. AIMP1 antibody dilution: 1:500.| Product Name | AIMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AIMP1 (aminoacyl tRNA synthetase complex-interacting multifunctional protein 1)The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rat, Pig, BovineRat (81%), Pig (85%), Bovine (83%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EMAP2 antibody, EMAPII antibody, SCYE1 antibody, p43 antibody, AIMP1 antibody, small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody, endothelial-monocyte activating polypeptide II antibody, aminoacyl tRNA synthase compl |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm , cytosol , Cytoplasmic vesicle , secretory vesicle , Secreted , Endoplasmic reticulum , Golgi apparatus |
Application
Sample(30 μg of whole cell lysate) A:H1299 B:Hep G2 12% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
AIMP1 antibody detects SCYE1 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. AIMP1 antibody dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
