Application
Western blot of recombinant mouse AIP using| Product Name | AIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AIPStudies have shown that the unactivated aryl hydrocarbon receptor (AhR) exists in a heterotetrameric 9S core with hsp90 and the aryl hydrocarbon receptor interacting protein (AIP, also known as ARA9 or XAP-2). AIP is a 330 amino acid protein that resembles the 52 kDa FKBP52 protein sequence, but does not share the same affinity for the hsp90-glucocorticoid receptor complex. It has been demonstrated through studies that AIP helps stabilize the AIP-hsp90-AhR complex and protects the AhR receptor from ubiquitination under heat stress conditions. These studies also suggest that AIP plays an important role in regulating the rate of AhR turnover by delaying AhR nuclear translocation after ligand binding. Immunopreciatation experiments have also shown that the N-terminus of AIP is required for the stability of the dioxin receptor-hsp90-AIP complex and the C-terminus is needed for direct contact and binding to hsp90. |
| Immunogen | Full length, bacterially expressed mouse recombinant AIP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:200 Optimal dilution / concentration should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIP, AA408703, AW476050, D19Bwg1412e, Ara9, Xap2, 11632 |
|---|---|
| Target | AIP |
Application
Western blot of recombinant mouse AIP using| Application Notes | WB: 1:200 Optimal dilution / concentration should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
