Application
HEK293T cells transfected with either RC204408 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK3 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK3 monoclonal antibody.| Product Name | AK3 antibody [5A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5A1] to AK3The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human AK3 (NP_057336) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5A1 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AKL3L1, AK3, AK6, AKL3L, AK3L1, FIX |
|---|---|
| Target | AK3 |
| Tissue Specificity | is specific for human AK3 (NP_057336). |
Application
HEK293T cells transfected with either RC204408 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK3 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK3 monoclonal antibody.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
