Application
HEK293T cells transfected with either RC222241 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK5 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.Application
Anti-AK5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AK5| Product Name | AK5 antibody [1C5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C5] to AK5Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. |
| Immunogen | Full length human recombinant protein of human AK5 (NP_777283) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1C5 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AK5, MGC33326, AK6 |
|---|---|
| Target | AK5 |
| Tissue Specificity | is specific for human AK5 (NP_777283). |
Application
HEK293T cells transfected with either RC222241 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK5 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.
Application
Anti-AK5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AK5| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
