Application
Immunofluorescence analysis of methanol-fixed HeLa, using AKAP10 antibody at 1:500 dilution.Application
AKAP10 antibody [C3], C-term detects AKAP10 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AKAP10 antibody [C3], C-term diluted at 1:100.Application
Immunohistochemical analysis of paraffin-embedded human OVCAR3, using AKAP10 antibody at 1:100 dilution.Application
AKAP10 antibody [C3], C-term detects AKAP10 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. AKAP10 antibody [C3], C-term diluted at 1:100.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKAP10 antibody at 1:100 dilution.| Product Name | AKAP10 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKAP10 (A kinase (PRKA) anchor protein 10)The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Mouse, RatMouse (85%), Rat (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D-AKAP2 antibody MGC9414 antibody PRKA10 antibody AKAP10 antibody dual specificity A kinase-anchoring protein 2 antibody D-AKAP-2 antibody a kinase anchor protein 10 mitochondrial antibody A-kinase anchor protein 10 mitochondrial antibody dual-sp |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) |
| Cellular Localization | Mitochondrion , Membrane , Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using AKAP10 antibody at 1:500 dilution.
Application
AKAP10 antibody [C3], C-term detects AKAP10 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AKAP10 antibody [C3], C-term diluted at 1:100.
Application
Immunohistochemical analysis of paraffin-embedded human OVCAR3, using AKAP10 antibody at 1:100 dilution.
Application
AKAP10 antibody [C3], C-term detects AKAP10 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. AKAP10 antibody [C3], C-term diluted at 1:100.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKAP10 antibody at 1:100 dilution.| Positive Control | Synthetic peptide: 1:8,000 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
