Rabbit polyclonal to AKAP3 (Internal)The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family, and is expressed in testis only. The encoded protein contains an RII-binding domain, and is predicted to participate in protein-protein interactions with the R-subunit of the PKA. This protein is localized to the ribs of the fibrous sheath in the principal piece of the sperm tail. It may function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. [provided by RefSeq]
Immunogen
KLH conjugated synthetic peptide between 246~276 amino acids from the Center region of human AKAP3.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
ELISA: Use at 1:1,000:
Species Reactivity
Human
Concentration
0.2mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
A-kinase anchor protein 3 AKAP110 Protein kinase A-anchoring protein 3 Protein kinase Aanchoring protein3 SOB1 Protein kinase Aanchoring protein 3 Akinase anchor protein110 kDa Fibrous sheath protein of 95 kDa Fibrous sheath protein of95 kDa Fibro
Target
AKAP3
Molecular Weight(MW)
94.736 kDa (note)
Cellular Localization
Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
Application
Application
Western blot analysis of AKAP3 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). AKAP3 (arrow) was detected using the purified Pab.
Application Notes
ELISA: Use at 1:1,000:
Additional Information
Form
Liquid
Storage Instructions
Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.