Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-AKR1A1mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-AKR1A1mouse monoclonal antibody. ( Dilution 1:50)Application
Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).| Product Name | AKR1A1 antibody [9F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9F1] to AKR1A1Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). |
| Immunogen | Full length human recombinant protein of human AKR1A1 (NP_006057) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 9F1 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC12529, MGC1380, ALR, ALDR1, AKR1A1 |
|---|---|
| Target | AKR1A1 |
| Tissue Specificity | is specific for human AKR1A1 (NP_006057). |
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-AKR1A1mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-AKR1A1mouse monoclonal antibody. ( Dilution 1:50)
Application
Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
