Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKR1B1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using AKR1B1 antibody at 1:200 dilution.Application
Wild-type (WT) and AKR1B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1B1 antibody [N1C3] diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000| Product Name | AKR1B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKR1B1 (aldo-keto reductase family 1, member B1 (aldose reductase))This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AKR1B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanPigRat |
| Predicted Reactivity | Dog, Rabbit, Rhesus Monkey, BovineDog (91%), Rabbit (89%), Rhesus Monkey (98%), Bovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADR antibody, ALDR1 antibody, ALR2 antibody, AR antibody, MGC1804 antibody, AKR1B1 antibody, aldehyde reductase 1 antibody, aldose reductase antibody, aldo-keto reductase family 1 member B1 antibody, low Km aldose reductase antibody, Lii5-2 CTCL tumor anti |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKR1B1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using AKR1B1 antibody at 1:200 dilution.
Application
Wild-type (WT) and AKR1B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1B1 antibody [N1C3] diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
