Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10 antibody at 1:200 dilution.Application
AKR1B10 antibody detects AKR1B10 protein by western blot analysis. A. 50 µg rat colon lysate/extract 10% SDS-PAGE AKR1B10 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: MOLT4 B: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
AKR1B10 antibody detects AKR1B10 protein by western blot analysis. A. 50 µg mouse colon lysate/extract 10% SDS-PAGE AKR1B10 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
AKR1B10 antibody detects AKR1B10 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 antibody diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10 antibody at 1:500 dilution.| Product Name | AKR1B10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKR1B10 (aldo-keto reductase family 1, member B10 (aldose reductase))This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq] |
| Immunogen | Full length human AKR1B10 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | RabbitRabbit (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKR1B11 antibody AKR1B12 antibody ALDRLn antibody ARL-1 antibody ARL1 antibody HIS antibody HSI antibody MGC14103 antibody AKR1B10 antibody aldo-keto reductase family 1 member B10 antibody hARP antibody small intestine reductase antibody aldose |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10 antibody at 1:200 dilution.
Application
AKR1B10 antibody detects AKR1B10 protein by western blot analysis. A. 50 µg rat colon lysate/extract 10% SDS-PAGE AKR1B10 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: MOLT4 B: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
AKR1B10 antibody detects AKR1B10 protein by western blot analysis. A. 50 µg mouse colon lysate/extract 10% SDS-PAGE AKR1B10 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
AKR1B10 antibody detects AKR1B10 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 antibody diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10 antibody at 1:500 dilution.| Positive Control | Molt-4 , Raji , mouse colon , rat colon , Jurkat , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
