Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1C3 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
AKR1C3 antibody [C2C3], C-term detects AKR1C3 protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3 antibody at 1:100 dilution.Application
Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term . Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | AKR1C3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKR1C3 (aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II))This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKR1C3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DD3 antibody DDX antibody HA1753 antibody HAKRB antibody HAKRe antibody HSD17B5 antibody KIAA0119 antibody PGFS antibody hluPGFS antibody AKR1C3 antibody aldo-keto reductase family 1 member C3 antibody type IIb 3-alpha hydroxysteroid dehydrogena |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1C3 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
AKR1C3 antibody [C2C3], C-term detects AKR1C3 protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3 antibody at 1:100 dilution.
Application
Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term . Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | HepG2 , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
