Application
Immunoprecipitation of Akt1/2/3 (phospho Ser473/474/472) protein from 293T whole cell extracts using 5 μg of Akt1/2/3 (phospho Ser473/474/472) antibody . Western blot analysis was performed using Akt1/2/3 (phospho Ser473/474/472) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Untreated (–) and treated (+) HUVEC whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MCF-7 cells were untreated or treated with 500 nM PMA for 1, 2, 4 and 6 hrs. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | AKT (phospho Ser473) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT(phospho Ser473) 3. Target: AKT(phospho Ser473)The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
| Modification | p-Ser473 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Cat, Pig, Chicken, Sheep, Rhesus MonkeyRat (100%), Zebrafish (100%), Xenopus laevis (100%), Cat (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKT antibody MGC99656 antibody PKB antibody PKB-ALPHA antibody PRKBA antibody RAC antibody RAC-ALPHA antibody AKT1 antibody protein kinase B alpha antibody rac protein kinase alpha antibody RAC-alpha serine/threonine-protein kinase antibody prot |
|---|---|
| Target | AKT(phospho Ser473) |
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Tissue Specificity | This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. |
| Cellular Localization | Cytoplasm , Nucleus , Cell membrane |
Application
Immunoprecipitation of Akt1/2/3 (phospho Ser473/474/472) protein from 293T whole cell extracts using 5 μg of Akt1/2/3 (phospho Ser473/474/472) antibody . Western blot analysis was performed using Akt1/2/3 (phospho Ser473/474/472) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Untreated (–) and treated (+) HUVEC whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MCF-7 cells were untreated or treated with 500 nM PMA for 1, 2, 4 and 6 hrs. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | MCF-7 , MCF-7(500 nM PMA treatment for 1 hr) , MCF-7(500 nM PMA treatment for 2 hr) , MCF-7(500 nM PMA treatment for 4 hr) , MCF-7(500 nM PMA treatment for 6 hr) , HUVEC , HUVEC (5 ng/ml VEGF treatment for 10 min) , NIH-3T3 , NIH-3T3 (starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
