Application
Western Blot of Rabbit anti-Akt phospho T308 antibody . Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.| Product Name | AKT (phospho Thr308) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT (phospho Thr308)Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. |
| Immunogen | Synthetic peptide: DGATMKT p FCG conjugated to KLH, corresponding to amino acids 302-311 of AKT. |
| Modification | p-Thr308 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Akt (phospho Thr308) |
|---|---|
| Cellular Localization | Cytoplasmic and Nuclear after activation by integrin linked protein kinase 1 (ILK1). |
Application
Western Blot of Rabbit anti-Akt phospho T308 antibody . Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
