Application
Western blot analysis of Akt phosphorylation expression in Insulin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.IF
a, c Representative immunofluorescence images of p-Akt and GAP 43 of neurons at the injured site. The white arrows indicate the positive cells.WB
(C–E) The phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473) and AKT (phospho Thr308) was markedly reduced by HG inducement.WB
(D, E) DOP treatment markedly relived HG-induced suppressive effects on the phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.WB
(C, D) IRS-1/2 inhibitor NT157 treatment notably reversed HG-induced increased phosphorylation of IRS-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.IHC
A, Hematoxylin and eosin staining (scale bar: 20 μm). B, Masson's trichrome staining (scale bar: 20 μm). C–F, Immunohistochemical staining to detect Hsp70 (C, scale bar: 20 μm), p-Akt (D, scale bar: 20 μm), p-JNK (E, scale bar: 20 μm), and p-ERK (F, scale bar: 20 μm) expression.WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium.| Product Name | AKT (phospho Thr308) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Akt (phospho Thr308)The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011] |
| Immunogen | A synthesized peptide derived from human Akt around the phosphorylation site of Thr308 |
| Modification | p-Thr308 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | phospho Akt (phospho Thr308) (pT308) Akt (phospho Thr308) T308 Phospho Akt (phospho Thr308) (phospho T308) RAC phospho Akt (phospho Thr308) (Thr308) Akt (phospho Thr308) (pT308) Phospho Akt (phos |
|---|---|
| Target | Akt (phospho Thr308) |
| Tissue Specificity | AKT1 |
Application
Western blot analysis of Akt phosphorylation expression in Insulin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
IF
a, c Representative immunofluorescence images of p-Akt and GAP 43 of neurons at the injured site. The white arrows indicate the positive cells.
WB
(C–E) The phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473) and AKT (phospho Thr308) was markedly reduced by HG inducement.
WB
(D, E) DOP treatment markedly relived HG-induced suppressive effects on the phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.
WB
(C, D) IRS-1/2 inhibitor NT157 treatment notably reversed HG-induced increased phosphorylation of IRS-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.
IHC
A, Hematoxylin and eosin staining (scale bar: 20 μm). B, Masson's trichrome staining (scale bar: 20 μm). C–F, Immunohistochemical staining to detect Hsp70 (C, scale bar: 20 μm), p-Akt (D, scale bar: 20 μm), p-JNK (E, scale bar: 20 μm), and p-ERK (F, scale bar: 20 μm) expression.
WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
