Application
AKT1-S129 antibody western blot analysis in Jurkat,A375,Y79 cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).| Product Name | AKT1 (phospho Ser129) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT1 (phospho Ser129)The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011] |
| Immunogen | Synthetic peptide between 107-135 amino acids from human AKT1. |
| Modification | p-Ser129 |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PKB-ALPHA , RAC , v-akt murine thymoma viral oncogene homolog 1 , CWS6 , AKT1 , PKB , PRKBA , AKT , RAC-ALPHA |
|---|---|
| Target | AKT1 (phospho Ser129) |
| Tissue Specificity | This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-135 amino acids from human AKT1. |
Application
AKT1-S129 antibody western blot analysis in Jurkat,A375,Y79 cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).| Application Notes | WB: 1:1000 |
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| Form | Liquid |
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| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
