Application
Untreated (–) and treated (+) HCF-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody diluted at 1:1000.| Product Name | AKT1 (phospho Ser129) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT1 (phospho Ser129) (v-akt murine thymoma viral oncogene homolog 1)The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human AKT1 (phospho Ser129). The exact sequence is proprietary. |
| Modification | p-Ser129 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Cat, Chicken, Rhesus MonkeyMouse (100%), Xenopus laevis (100%), Cat (100%), Chicken (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | AKT1 (phospho Ser129) |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus , Cell membrane |
Application
Untreated (–) and treated (+) HCF-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
