Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1 antibody at 1:250 dilution.Application
Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody diluted at 1:5000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE diluted at 1:10000Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Akt1 antibody detects Akt1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | AKT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT1 (v-akt murine thymoma viral oncogene homolog 1)The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Akt1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Cat, Pig, Chicken, Sheep, Rhesus Monkey, BovineRat (97%), Zebrafish (84%), Xenopus laevis (91%), Cat (98%), Pig (96%), Chicken (96%), Sheep (96%), Rhesus Monkey (99%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1 antibody, protein kinase B alpha antibody, rac protein kinase alpha antibody, RAC-alpha serine/threonine-protein kinase antibody, prot |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
| Cellular Localization | Cytoplasm , Nucleus , Cell membrane |
Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1 antibody at 1:250 dilution.
Application
Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody diluted at 1:5000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE diluted at 1:10000
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Akt1 antibody detects Akt1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
