Application
Immunofluorescence staining of methanol-fixed Hela cells using AKT1 Antibody .Application
Western blot analysis of extracts from 293 and MCF cells using AKT1 Antibody .Application
Western blot analysis of extracts from 293 and MCF cells using AKT1(Ab-450) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | AKT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT1General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. |
| Immunogen | Peptide sequence around aa.448~452 (T-I-T-P-P) derived from Human AKT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1, protein kinase B alpha, rac protein kinase alpha, RAC-alpha serine/threonine-protein kinase, proto-oncogene c-Akt, RAC-PK-alpha, PKB alpha, v-akt murine thymoma viral oncogene homolog 1 |
|---|---|
| Target | AKT1 |
| Molecular Weight(MW) | 60 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using AKT1 Antibody .
Application
Western blot analysis of extracts from 293 and MCF cells using AKT1 Antibody .
Application
Western blot analysis of extracts from 293 and MCF cells using AKT1(Ab-450) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
