Application
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using Akt2(Phospho-Ser474)Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Phospho-Ser474) (left) or the same antibody preincubated with blocking peptide(right).| Product Name | AKT2 (phospho Ser474) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Akt2 (Phospho Ser474)General protein kinase capable of phosphorylating several known proteins. |
| Immunogen | Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2. |
| Modification | p-Ser474 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PKBB, PKBBETA, PRKBB, RAC-BETA, AKT2, protein kinase B beta, murine thymoma viral (v-akt) homolog-2, protein kinase Akt-2, RAC-PK-beta, PKB beta, rac protein kinase beta, RAC-beta serine/threonine-protein kinase, v-akt murine thymoma viral oncogene homolog |
|---|---|
| Target | Akt2 (Phospho Ser474) |
| Molecular Weight(MW) | 60 (note) |
Application
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using Akt2(Phospho-Ser474)
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Phospho-Ser474) (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
