Application
AKT2 antibody detects AKT2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. AKT2 antibody diluted at 1:400.Application
Akt2 antibody detects Akt2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells 7.5 % SDS-PAGE Akt2 antibody dilution: 1:20000Application
Akt2 antibody detects Akt2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 7.5 % SDS-PAGE Akt2 antibody dilution: 1:1000| Product Name | AKT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT2 (v-akt murine thymoma viral oncogene homolog 2)This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Akt2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PKBB antibody , PKBBETA antibody , PRKBB antibody , RAC-BETA antibody , AKT2 antibody, protein kinase B beta antibody, murine thymoma viral (v-akt) homolog-2 antibody, protein kinase Akt-2 antibody, RAC-PK-beta antibody, PKB beta antibody, rac pr |
|---|---|
| Target | AKT2 |
| Molecular Weight(MW) | 56 kDa (note) (4) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
Application
AKT2 antibody detects AKT2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. AKT2 antibody diluted at 1:400.
Application
Akt2 antibody detects Akt2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells 7.5 % SDS-PAGE Akt2 antibody dilution: 1:20000
Application
Akt2 antibody detects Akt2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 7.5 % SDS-PAGE Akt2 antibody dilution: 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
