Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000Application
Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Akt3 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: Rat brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| Product Name | AKT3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AKT3 (v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma))The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Akt3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, Chimpanzee, BovinePig (99%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434N0250 antibody PKB-GAMMA antibody PKBG antibody PRKBG antibody RAC-PK-gamma antibody RAC-gamma antibody STK-2 antibody AKT3 antibody PKB gamma antibody RAC-gamma serine/threonine-protein kinase antibody RAC-gamma serine/threonine protein |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Membrane |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000
Application
Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Akt3 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: Rat brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| Positive Control | HepG2 , HA-AKT transfected HeLa cell , mouse brain , Rat brain , *SD rat brain cotex |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
