Application
Titration of human serum in two albumin sandwich immunoassays. Human Albumin antibody [15C7] for capture and Human Albumin antibody [1A9] for detection| Product Name | Albumin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1A9] to AlbuminSerum albumin, a globular unglycosylated protein of molecular weight 65,000, is the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin. |
| Immunogen | Human serum albumin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1A9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 65 kDa. Can be paired for ELISA with GTX10241 Mouse monoclonal [15C7] to Albumin . Produces sensitive assay system with rapid kinetics (10-15 mins in plate format) for the detection of albumin in human urine. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | PRO1341 DKFZp779N1935 Human Albumin ALB P02768 213 PRO0903 103600 PRO0883 |
|---|---|
| Target | Albumin |
| Tissue Specificity | No cross-reactivity with other human proteins tested. |
| Cellular Localization | Secreted |
Application
Titration of human serum in two albumin sandwich immunoassays. Human Albumin antibody [15C7] for capture and Human Albumin antibody [1A9] for detection| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 65 kDa. Can be paired for ELISA with GTX10241 Mouse monoclonal [15C7] to Albumin . Produces sensitive assay system with rapid kinetics (10-15 mins in plate format) for the detection of albumin in human urine. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
