Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH1A3 antibody at 1:200 dilution.Application
Immunofluorescence analysis of methanol-fixed A549, using ALDH1A3 antibody at 1:500 dilution.| Product Name | ALDH1A3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALDH1A3 (aldehyde dehydrogenase 1 family, member A3)Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus MonkeyMouse (95%), Rat (95%), Xenopus laevis (83%), Chicken (88%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALDH1A6 antibody ALDH6 antibody RALDH3 antibody ALDH1A3 antibody RALDH-3 antibody aldehyde dehydrogenase 6 antibody retinaldehyde dehydrogenase 3 antibody aldehyde dehydrogenase family 1 member A3 antibody acetaldehyde dehydrogenase 6 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH1A3 antibody at 1:200 dilution.
Application
Immunofluorescence analysis of methanol-fixed A549, using ALDH1A3 antibody at 1:500 dilution.| Positive Control | A549 , HepG2 , HCT116 |
|---|---|
| Application Notes | WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
