Application
ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ALDH7A1 antibody [C1C3] dilution: 1:500.Application
ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ALDH7A1 protein stained by ALDH7A1 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH7A1 antibody [C1C3] diluted at 1:3000.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE ALDH7A1 antibody diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:10000| Product Name | ALDH7A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALDH7A1 (aldehyde dehydrogenase 7 family, member A1)Antiquitin is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. Mutations in this gene cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride. Four additional human antiquitin-like sequences, all of which are pseudogenes, have also been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH7A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, BovineZebrafish (82%), Xenopus laevis (81%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATQ1 antibody EPD antibody FLJ11738 antibody FLJ92814 antibody PDE antibody ALDH7A1 antibody antiquitin-1 antibody betaine aldehyde dehydrogenase antibody P6c dehydrogenase antibody alpha-aminoadipic semialdehyde dehydrogenase antibody alpha-AASA |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (3) |
Application
ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ALDH7A1 antibody [C1C3] dilution: 1:500.
Application
ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: ALDH7A1 protein stained by ALDH7A1 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH7A1 antibody [C1C3] diluted at 1:3000.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE ALDH7A1 antibody diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:10000| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse liver , rat liver |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
