Application
Immunofluorescence analysis of methanol-fixed A431, using Aldolase B antibody at 1:200 dilution.Application
Aldolase B antibody detects ALDOB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE Aldolase B antibody dilution: 1:1000Application
Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:10000Application
Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. Aldolase B antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: JurKat D: Raji 10% SDS PAGE diluted at 1:1000| Product Name | Aldolase B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Aldolase B (aldolase B, fructose-bisphosphate)Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Rabbit, Chicken, Sheep, BovineZebrafish (82%), Rabbit (98%), Chicken (84%), Sheep (93%), Bovine (94%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALDB antibody ALDO2 antibody ALDOB antibody fructose-bisphosphate aldolase B antibody aldolase 2 antibody aldolase B fructose-bisphosphatase antibody liver-type aldolase antibody aldolase B fructose-bisphosphate antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed A431, using Aldolase B antibody at 1:200 dilution.
Application
Aldolase B antibody detects ALDOB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE Aldolase B antibody dilution: 1:1000
Application
Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:10000
Application
Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. Aldolase B antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: JurKat D: Raji 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji , mouse liver , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
