Application
Immunohistochemistry of ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 μg/mL.Application
Western blot analysis of ALKBH2 in human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 ug/ml.| Product Name | ALKBH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ALKBH2The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | alkylation repair homolog 2 ALKBH2 alkylation repair homolog2 ABH2 Alpha-ketoglutarate-dependent dioxygenase alkB homolog2 Alphaketoglutaratedependent dioxygenase alkB homolog2 |
|---|---|
| Target | ALKBH2 |
| Tissue Specificity | This antibody is specific for ALKBH2 |
Application
Immunohistochemistry of ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 μg/mL.
Application
Western blot analysis of ALKBH2 in human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
