Application
Immunohistochemistry of ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 μg/mL.Application
Western blot analysis of ALKBH2 in human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ALKBH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALKBH2 Antibody: ABH2, ABH2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Alkylated DNA repair protein alkB homolog 2 |
| Product description | ALKBH2 Antibody: The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.1) Duncan T, Trewick SC, Koivisto P, et al. Reversal of DNA alkylation damage by two human dioxygenases. Proc. Natl. Acad. Sci. USA2002; 99:16660-5. |
| Immunogen | ALKBH2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ALKBH2. The immunogen is located within amino acids 180 - 230 of ALKBH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ALKBH2 antibody can be used for detection of ALKBH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALKBH2 |
|---|---|
| Alternative Names | ALKBH2 Antibody: ABH2 ABH2 Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 Alkylated DNA repair protein alkB homolog 2 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Entrez Gene | 121642 |
|---|---|
| Protein Accession | NP_001138847 |
Application
Immunohistochemistry of ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 μg/mL.
Application
Western blot analysis of ALKBH2 in human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | ALKBH2 antibody can be used for detection of ALKBH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ALKBH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ALKBH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
