Application
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DEPC-1 antibody diluted at 1:1000.| Product Name | ALKBH3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALKBH3 (alkB, alkylation repair homolog 3 (E. coli))The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DEPC-1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Rat |
| Predicted Reactivity | Human, Rhesus MonkeyHuman (100%), Rhesus Monkey (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DEPC1 alkB homolog 3 alpha-ketoglutarate-dependent dioxygenase ABH3 ALKBH3 PCA1 |
|---|---|
| Target | DEPC-1 |
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DEPC-1 antibody diluted at 1:1000.| Positive Control | rat stomach |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
