Application
Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ALKBH8 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ALKBH8 (C-term)Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. |
| Immunogen | KLH conjugated synthetic peptide between 501~531 amino acids from the C-terminal region of human ALKBH8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Alkylated DNA repair protein alkB homolog8 Alkylated DNA repair protein alkB homolog 8 ALKBH8 |
|---|---|
| Target | ALKBH8 |
| Molecular Weight(MW) | 75.208 kDa (note) |
| Cellular Localization | Predominantly cytoplasmic. |
Application
Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
