Application
WB analysis of lysates from HEK293T cells transfected with the pCMV6-ENTRY control (left) or pCMV6-ENTRY SERPINA1 (right) cDNA for 48 hrs using alpha 1 Antitrypsin antibody [1C2].Application
HEK293T cells transfected with either A1AT overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-A1AT antibody , and then analyzed by flow cytometryApplication
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-A1AT mouse monoclonal antibody.Application
WB analysis of indicated extracts (35ug per lane) using alpha 1 Antitrypsin antibody [1C2].| Product Name | alpha 1 Antitrypsin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C2] to alpha 1 AntitrypsinInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 26-418 of human A1AT |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1C2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PWB |
| IHC 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AAT PI1 MGC23330 PRO2275 SERPINA1 A1AT MGC9222 PI A1A |
|---|---|
| Target | alpha 1 Antitrypsin |
| Tissue Specificity | is specific for amino acids 26-418 of human A1AT |
Application
WB analysis of lysates from HEK293T cells transfected with the pCMV6-ENTRY control (left) or pCMV6-ENTRY SERPINA1 (right) cDNA for 48 hrs using alpha 1 Antitrypsin antibody [1C2].
Application
HEK293T cells transfected with either A1AT overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-A1AT antibody , and then analyzed by flow cytometry
Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-A1AT mouse monoclonal antibody.
Application
WB analysis of indicated extracts (35ug per lane) using alpha 1 Antitrypsin antibody [1C2].| Application Notes | IHC 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
