Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-A1AT monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A1AT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-A1AT.| Product Name | alpha 1 Antitrypsin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3C5] to alpha 1 AntitrypsinInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 26-418 of human A1AT |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3C5 |
| Host Species | Mouse |
| Tested Applications | WB |
| IHC 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AAT PI1 MGC23330 PRO2275 SERPINA1 A1AT MGC9222 PI A1A |
|---|---|
| Target | alpha 1 Antitrypsin |
| Tissue Specificity | is specific for amino acids 26-418 of human A1AT |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-A1AT monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A1AT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-A1AT.| Application Notes | IHC 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
