Application
Rat embryonic brain (S1) extract was separated on SDS-PAGE and probed with IGG FRACTION AB TO α-E-CATENIN (RB) . The antibody was developed with HRP Anti-Rabbit IgG and an ECL substrate. Antibody dilution 1/2,000.| Product Name | alpha 1 Catenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to alpha 1 CateninThe catenins (alpha, beta and gamma) are cytoplasmic proteins found in varying abundance in many developing and adult tissues. Catenins bind directly or indirectly to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The association of catenins to cadherins produces a complex, which is linked to the actin filament network. Catenins/cadherin complexes play an important role in mediating cell adhesion, transduction of cell-cell contact positional signals to the cell interior, and may play a crucial role in cell differentiation. The linkage of the epithelial E-cadherin/ uvomorulin to actin is essential for the cell binding function of this cadherin. Alpha-Catenin (CAP102, 102 kDa), originally described as an E-cadherin associated protein, has been shown to associate with other members of the cadherin family members, N-cadherin and P-cadherin. |
| Immunogen | Synthetic peptide: EKQDETQTKIKRASQ conjugated to KLH, corresponding to amino acids 873-887 of Human alpha 1 Catenin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC: Use at a dilution of 1/100. WB: Use at a dilution of 1/1000. Detects a band of approximately 102 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseXenopus |
| Concentration | 1mg/ml |
| Gene Synonyms | 1495 FLJ36832 CTNNA1 CAP102 alpha 1 Catenin alpha1 Catenin 116805 P35221 alpha catenin antibody alpha catenin |
|---|---|
| Target | alpha 1 Catenin |
| Tissue Specificity | Anti-alpha-E-Catenin recognizes alpha-E-catenin (102 kDa).Staining of the alpha-E-catenin band in immunoblotting is specifically inhibited with the alpha-E-catenin immunizing peptide (human, amino acids 873-887). |
| Cellular Localization | Cell Membrane and Cytoplasmic |
Application
Rat embryonic brain (S1) extract was separated on SDS-PAGE and probed with IGG FRACTION AB TO α-E-CATENIN (RB) . The antibody was developed with HRP Anti-Rabbit IgG and an ECL substrate. Antibody dilution 1/2,000.| Application Notes | ICC: Use at a dilution of 1/100. WB: Use at a dilution of 1/1000. Detects a band of approximately 102 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
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| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
